Not a line, but in Ratatouille there's a point where Linguini is trying to explain to his love interest that he's being guided by a rat in his hat and he's saying, 'I've got a tiny... little...' We see the reaction shot of her looking confused/disgusted and very quickly glancing down at his crotch.
It's just a fraction of a second, but a great gag for the grownups anyway!
I’ve been forced to watch Frozen about a million times over the last few months. So the one that sticks out in my mind is when Kristoff asks Anna if she knows Hans’ foot size, and she just says “foot size doesn’t matter.”
Wish I could find a clip to post, but there's a Garfield special ("Babes and Bullets") themed as a black and white detective movie, and the line "Being a private dick isn't easy with a name like Sam Spayed" is forever burned into my memory.
Not my 'favorite' as I think Ron Howard is a hack and strongly disliked his retcon moralizing of Seuss's story, but it's pretty wild that the live action The Grinch has a reference to a key party (i.e. swapping sex partners based on what key you get) as the baby Grinch arrives down from the sky.
Ralph Bakshi is my hero. I was a teenager in the mid ‘80s when he did The New Adventures of Mighty Mouse on CBS Saturday morning. No one could understand why I was such a big fan of a kid’s cartoon, but it was pretty subversive for Saturday morning!