Not taking care of my health. Too much sitting in front of a computer, not enough walking around. Too much junk food.
Not keeping track of people after leaving their immediate circle (team changes, company changes, leaving college, etc.). Literally every opportunity I've had has come from somebody I know, yet I've done a poor job of keeping a network socially. It's not that hard to chat with people every few months, but I didn't initially put enough effort into it.
Happy:
For me specifically, staying at my first job for a long time was really good. It helped me grow, and the company was pretty good with salary increases.
In contrast to my regret, I did a good job of making friends with teammates and getting along with people I work with the most.