Protocol for Cloning

Ah, you cloned the wrong resistance gene again...

I fondly remember my times cloning vectors... Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.

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Meh, amateur. Didn’t check the PCR reaction on a gel first. Also didn’t add DNA ligase.
Ah, you cloned the wrong resistance gene again...
I fondly remember my times cloning vectors... Not. My favourite was a multistep cloning and digesting protocol that I meticulously planned. It obviously failed numerous times. I got it right on the first try just making blunt ends and hoping it goes in correctly.